Objectives
\nKlebsiella pneumoniae is an important emerging pathogen in humans and animals with serious clinical consequences. The increased use of antibiotics has promoted the emergence of carbapenem-resistant and \u03b2-lactamase (ESBL)-producing K. pneumoniae strains. Recently, phage therapy has gained momentum as a potential alternative against emerging antimicrobial resistance. This study was conducted to evaluate the therapeutic effects of a novel lytic phage (VTCCBPA43) in a pneumonic mouse model to investigate the efficacy of phage therapy against a virulent K. pneumoniae infection. <\/p>\n
Methods
\nThe tailed phage VTCCBPA43 was investigated regarding its growth kinetics, its in vitro host range, and its temperature and pH sensitivity. The protein components were analyzed using SDS-PAGE and nLC-MS\/MS. Therapeutic efficacy was observed 2 h post-challenge with virulent K. pneumoniae in a BALB\/c mouse model. <\/p>\n
Results
\nThe phage VTCCBPA43 proved to be highly temperature-tolerant (up to 80\u00b0C). It was most active at pH 5, had a burst size of 172 PFU\/mL, and exhibited a narrow host range. It was identified as a KP36-like phage through shotgun proteomics. Following intranasal administration of a single dose (2 \u00d7 10^9 PFU\/mouse) after infection with virulent K. pneumoniae, the presence of a biologically active phage in vivo and a significant reduction in the bacterial load of the lungs were observed at all time points. A reduction in lesion severity indicated an overall positive effect of VTCCBPA43 phage therapy in the pneumonic mouse model. <\/p>\n
Translation of the source: https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2213716519302516?via%3Dihub<\/p>\n","protected":false},"excerpt":{"rendered":"
Objectives Klebsiella pneumoniae is an important emerging pathogen in humans and animals with serious clinical consequences. The increased use of antibiotics has promoted the emergence of carbapenem-resistant and \u03b2-lactamase (ESBL)-producing K. pneumoniae strains. Recently, phage therapy has gained momentum as a potential alternative against emerging antimicrobial resistance. This study was conducted to evaluate the therapeutic […]<\/p>\n","protected":false},"author":1,"featured_media":8343,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[],"tags":[],"class_list":["post-8605","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry"],"jetpack_featured_media_url":"https:\/\/i0.wp.com\/www.phage.help\/wp-content\/uploads\/2020\/03\/maus.jpg?fit=480%2C341&ssl=1","jetpack_shortlink":"https:\/\/wp.me\/pazElU-2eN","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/www.phage.help\/en\/wp-json\/wp\/v2\/posts\/8605","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.phage.help\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.phage.help\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.phage.help\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.phage.help\/en\/wp-json\/wp\/v2\/comments?post=8605"}],"version-history":[{"count":0,"href":"https:\/\/www.phage.help\/en\/wp-json\/wp\/v2\/posts\/8605\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.phage.help\/en\/wp-json\/wp\/v2\/media\/8343"}],"wp:attachment":[{"href":"https:\/\/www.phage.help\/en\/wp-json\/wp\/v2\/media?parent=8605"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.phage.help\/en\/wp-json\/wp\/v2\/categories?post=8605"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.phage.help\/en\/wp-json\/wp\/v2\/tags?post=8605"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}