{"id":8698,"date":"2021-11-25T18:53:13","date_gmt":"2021-11-25T17:53:13","guid":{"rendered":"https:\/\/www.phage.help\/unkategorisiert\/one-step-closer-to-phage-therapy-for-antibiotic-resistant-infections\/"},"modified":"2021-11-25T18:53:13","modified_gmt":"2021-11-25T17:53:13","slug":"one-step-closer-to-phage-therapy-for-antibiotic-resistant-infections","status":"publish","type":"post","link":"https:\/\/www.phage.help\/en\/unkategorisiert\/one-step-closer-to-phage-therapy-for-antibiotic-resistant-infections\/","title":{"rendered":"One Step Closer to Phage Therapy for Antibiotic-Resistant Infections"},"content":{"rendered":"

Most phage therapy experiments begin and end in vitro\u2014in cultures of target bacteria. Such conditions have little to do with the actual situation in humans and animals. Therefore, scientists* have created a model that can simulate the microenvironment of the human body where bacterial colonization actually occurs. Experiments using this model have shown that Escherichia coli colonies do not develop resistance to bacteriophages under organismal conditions, as often occurs “in the test tube,” and phages successfully destroy most target microorganisms. <\/span><\/p>\n

\n

Due to the rapid spread of bacterial strains resistant to modern antimicrobial agents, scientists and clinicians are paying increasing attention to bacteriophages\u2014bacterial viruses that represent one of the effective and safe alternatives to antibiotics. Many cases of successful treatment of antibiotic-resistant infections with bacteriophages have been described. However, due to a number of limitations in testing and use, phages are not widely used in most Western countries. One of the problems is related to the difficulty of reproducing phage behavior in an organism under laboratory conditions. Experiments typically begin by studying the effect of phages on bacterial cultures in Petri dishes. Here, bacteria interact with each other, develop rapidly, and develop resistance to the tested phages. However, Petri dishes do not reproduce the conditions <\/span><\/p>\n

Scientists at the University of Exeter have* developed a method to reproduce this microenvironment in which a single bacterium can colonize a specific area. Here it does not mix with many other bacteria; here it encounters a bacteriophage specific to it. Using a new method, scientists have found that <\/span>Escherichia coli<\/span><\/em>, a bacterium that frequently causes food poisoning, does not acquire genetic resistance to phages in such a microenvironment, and phages are able to destroy most of the colony.<\/span><\/p>\n

Study leader Dr. Stefano Pagliara noted that antibiotic resistance could become a worse killer than COVID-19 if we do not find new ways to combat bacterial infections. In his opinion, phage therapy has great potential, and once it becomes part of routine clinical practice, it can save thousands of lives. <\/span><\/p>\n

The article published in the journal PLoS Biology lays the foundation for understanding how the environment affects the interaction between phages and bacteria, which is essential for developing effective phage therapy medications.<\/span><\/p>\n

The study also showed that some bacteria survive in the microenvironment without acquiring genetic resistance to phages. These bacteria survive due to having fewer phage receptors on their surface. The authors of the work point out that by increasing the number of phage receptors on the bacterial surface, the effectiveness of phage therapy can be enhanced. Therefore, it is necessary to search for ways to increase the number of receptors. <\/span><\/p>\n

* Attrill EL, Claydon R, \u0141api\u0144ska U, Recker M et al. Individual bacteria in structured environments rely on phenotypic resistance to phage. PLOS Biology, 2021; 19 (10): e3001406.<\/span>Doi: 10.1371\/journal.pbio.3001406<\/span><\/a><\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Most phage therapy experiments begin and end in vitro\u2014in cultures of target bacteria. Such conditions have little to do with the actual situation in humans and animals. Therefore, scientists* have created a model that can simulate the microenvironment of the human body where bacterial colonization actually occurs. Experiments using this model have shown that Escherichia […]<\/p>\n","protected":false},"author":1,"featured_media":8699,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"footnotes":""},"categories":[],"tags":[],"class_list":["post-8698","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry"],"jetpack_featured_media_url":"https:\/\/www.phage.help\/wp-content\/uploads\/2021\/11\/Mikrobiologie.jpg","_links":{"self":[{"href":"https:\/\/www.phage.help\/en\/wp-json\/wp\/v2\/posts\/8698","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.phage.help\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.phage.help\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.phage.help\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.phage.help\/en\/wp-json\/wp\/v2\/comments?post=8698"}],"version-history":[{"count":0,"href":"https:\/\/www.phage.help\/en\/wp-json\/wp\/v2\/posts\/8698\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.phage.help\/en\/wp-json\/wp\/v2\/media\/8699"}],"wp:attachment":[{"href":"https:\/\/www.phage.help\/en\/wp-json\/wp\/v2\/media?parent=8698"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.phage.help\/en\/wp-json\/wp\/v2\/categories?post=8698"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.phage.help\/en\/wp-json\/wp\/v2\/tags?post=8698"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}